Characterization of the truncated hemoglobin THB1 from protein extracts of Chlamydomonas reinhardtii
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چکیده
منابع مشابه
Characterization of the truncated hemoglobin THB1 from protein extracts of Chlamydomonas reinhardtii
Truncated hemoglobins (TrHbs) belong to the hemoglobin superfamily, but unlike their distant vertebrate relatives, little is known about their principal physiologic functions. Several TrHbs have been studied in vitro using engineered recombinant peptides. These efforts have resulted in a wealth of knowledge about the chemical properties of TrHbs and have generated interesting functional leads...
متن کاملCharacterization of the truncated hemoglobin THB1 from protein
Truncated hemoglobins (TrHbs) belong to the hemoglobin superfamily, but unlike their distant vertebrate relatives, little is known about their principal physiologic functions. Several TrHbs have been studied using in vitro engineered recombinant peptides. These efforts have resulted in a wealth of knowledge about the chemical properties of TrHbs and have generated interesting functional lea...
متن کاملCharacterization of THB1, a Chlamydomonas reinhardtii Truncated Hemoglobin: Linkage to Nitrogen Metabolism and Identification of Lysine as the Distal Heme Ligand
The nuclear genome of the model organism Chlamydomonas reinhardtii contains genes for a dozen hemoglobins of the truncated lineage. Of those, THB1 is known to be expressed, but the product and its function have not yet been characterized. We present mutagenesis, optical, and nuclear magnetic resonance data for the recombinant protein and show that at pH near neutral in the absence of added liga...
متن کاملFunctional and Spectroscopic Characterization of Chlamydomonas reinhardtii Truncated Hemoglobins
The single-cell green alga Chlamydomonas reinhardtii harbors twelve truncated hemoglobins (Cr-TrHbs). Cr-TrHb1-1 and Cr-TrHb1-8 have been postulated to be parts of the nitrogen assimilation pathway, and of a NO-dependent signaling pathway, respectively. Here, spectroscopic and reactivity properties of Cr-TrHb1-1, Cr-TrHb1-2, and Cr-TrHb1-4, all belonging to clsss 1 (previously known as group N ...
متن کاملTruncated hemoglobin 1 is a new player in Chlamydomonas reinhardtii acclimation to sulfur deprivation
Truncated hemoglobins constitute a large family, present in bacteria, in archaea and in eukaryotes. However, a majority of physiological functions of these proteins remains to be elucidated. Identification and characterization of a novel role of truncated hemoglobins in the model alga provides a framework for a more complete understanding of their biological functions. Here, we use quantitative...
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ژورنال
عنوان ژورنال: F1000Research
سال: 2014
ISSN: 2046-1402
DOI: 10.12688/f1000research.5873.1